Work

The Effect and Mechanism of 5-ALA PDT in Cervical Intraepithelial Neoplasia and Cervical Cancer

Year: 2025

Type: article

Abstract: Significance Cervical cancer is a major global health issue for women. 5-Aminolevulinic acid-mediated photodynamic therapy (5-ALA PDT) shows promise but has variable efficacy, necessitating in - depth study of its mechanisms and optimization strategies. Approach CCK-8 assay, flow cytometry, Western blot, real-time PCR analysis and siRNA were used to study the effects of 5-ALA PDT on H8 and Hela cells, including anti-proliferative, apoptosis, cell cycle, and gene expression. Results 5-ALA PDT inhibited the growth of H8 and Hela cells, induced apoptosis, blocked the cell cycle at G0/G1 phase, up-regulated p53, p21, RbAp48, and down - regulated HPV16 oncoproteins E6 and E7. Reduction of RbAp48 weakened the effects of 5-ALA PDT. Conclusions RbAp48 enhances the sensitivity of H8 cells to 5-ALA PDT through multiple mechanisms, which is crucial for understanding and optimizing 5-ALA PDT in treating cervical cancer.

Source: Photodiagnosis and Photodynamic Therapy

DOI: 10.1016/j.pdpdt.2025.105051

Authors: Yi Li, Youzhong Zhang, Shuxia Wu, Kan Jiang, Hongli Liu

Institutions: Shandong University, Qilu Hospital of Shandong University

Language: English

Topic: Photodynamic Therapy Research Studies

Subfield: Pulmonary and Respiratory Medicine

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $2,610