Work

DHA induces ferroptosis to enhance the efficacy of the photodynamic treatment of glioma through inhibits the Nrf2-Keap1 pathway

Year: 2025

Type: article

Abstract: Objective Explore the effects of DHA combined with PDT on ferroptosis and ROS production in glioma cells. Methods The activity of tumor cells was detected by MTT assay. The invasion and proliferation ability of tumor cells were detected by scratch and transwell. ROS production was detected by confocal microscopy and flow cytometry. In vitro and in vivo experiments were conducted to verify the effect of the screening pathway on ferroptosis and ROS production in glioma. Results In vivo and in vitro experiments showed that combined therapy could produce a significant inhibitory effect on glioma. Through bioinformatics analysis, Nrf2 was selected as a gene related to ferroptosis for further study. DHA combined with PDT can induce ferroptosis and ROS production by inhibiting the Nrf2-Keap1 pathway. Conclusion DHA combined with PDT can induce ferroptosis and ROS production in glioma by inhibiting the Nrf2-Keap1 pathway, and inhibiting the invasion and proliferation of glioma.

Source: Photodiagnosis and Photodynamic Therapy

DOI: 10.1016/j.pdpdt.2025.104831

Authors: Xiuwei Yan, Shaoshan Hu

Institutions: Zhejiang Provincial People's Hospital, Hangzhou Medical College

Language: English

Topic: Ferroptosis and cancer prognosis

Subfield: Pulmonary and Respiratory Medicine

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $2,610