Work

Ultraviolet Radiation – C in decontamination of organ during hypothermic perfusion

Year: 2025

Type: article

Abstract: Significance Bacterial properties of 254nm (ultraviolet-C) have been explored in organ decontamination ex vivo during hypothermic perfusion for Staphylococcus aureus , a disseminated gram-positive pathogen. By combining Ps80%+UV-C, full reduction (perfusate) and 2-log reduction (macerate) are achieved. Our aim is to verify these results for Escherichia coli , a common gram-negative urinary pathogen. Approach E. coli (ATCC25922) strain was used to contaminate porcine kidneys ex vivo . After harvesting, the kidneys were cannulated and perfused with a UV-C decontamination system, and 10% PS80 was added to HTK solution for 4 h. During and after experimentation, samples were collected for analysis. Results Results indicate no bacterial growth in samples collected after 70 min of UV-C irradiation whereas growth was consistent with initial inoculated load for control groups. Kidney macerate revealed a 99% reduction in bacterial load compared to control. Conclusions This study verified the effectiveness of the decontamination process for E. coli.

Source: Photodiagnosis and Photodynamic Therapy

DOI: 10.1016/j.pdpdt.2025.104974

Authors: L.C. Goenaga-Mafud, J.D. Vollet-Filho, J.N. Croce, N.M. Inada, C. Kurachi, V.S. Bagnato

Institution: Universidade de São Paulo

Language: English

Topic: Photodynamic Therapy Research Studies

Subfield: Pulmonary and Respiratory Medicine

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $2,610