Work

Effects of Photodynamic Inactivation on Susceptibility of methicillin-resistant Staphylococcus aureus (MRSA) in Mixed Culture

Year: 2025

Type: article

Abstract: Staphylococcus aureus and methicillin-resistant Staphylococcus aureus (MRSA) are bacteria of extreme concern to public health, as they are intrinsically related to hospital infections and difficulty in treating them. Bacteria have a pattern of behavior observed in survival curves. Mixed cultures behave differently from pure cultures and when subjected to photodynamic inactivation (PDI) treatment there are effects resulting in pathogenic and resistant bacteria becoming more susceptible. Photodynamic inactivation treatment was carried out using a stock solution (5 mM) of curcumin as a photosensitizer, irradiation with an LED lighting device (Biotable - produced by MM Optics-Brasil) emitting a wavelength of 450 nm and varying light doses between 1 J/cm², 2 J/cm² and 5 J/cm². By adding competing bacteria such as Staphylococcus epidermidis , it is possible to observe promising patterns for the treatment of resistant bacterial infections with PDI, such as a bacterial reduction of 3 log.

Source: Photodiagnosis and Photodynamic Therapy

DOI: 10.1016/j.pdpdt.2025.105049

Authors: Rebeca Vieira de Lima, Kate Cristina Blanco, Vanderlei Salvador Bagnato

Institutions: Universidade Federal de São Carlos, Universidade de São Paulo, Instituto de Física de São Carlos, Texas A&M University

Language: English

Topic: Photodynamic Therapy Research Studies

Subfield: Pulmonary and Respiratory Medicine

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $2,610