Work

Growing new teeth: A systematic review of functional whole-tooth regeneration in orthotopic animal models

Year: 2025

Type: review

Abstract: To identify and evaluate current strategies for functional whole-tooth regeneration in orthotopic animal models with high translational potential. A systematic review of in vivo studies involving orthotopic implantation of generated tooth constructs, analyzing experimental design, cell sources, scaffolding materials, implantation techniques, and outcome measures including histological, radiological, and functional evaluations. Considerable heterogeneity was observed in animal models, developmental stages at implantation, and cellular components. Polycaprolactone (PCL) membranes were often associated with improved eruption and reduced ankylosis, suggesting enhanced periodontal integration. PCL-based scaffolding systems may facilitate functional tooth regeneration, but further standardized in vivo research is needed to validate translational potential. • Autologous tooth germ transplants showed consistent functional success; xenogeneic methods lacked the ability to erupt. • Polycaprolactone (PCL) membranes improved eruption rates, reduced ankylosis, and enhanced periodontal integration. • Earlier developmental stages at implantation were associated with better structural and functional outcomes. • Enamel regeneration remains difficult; success occurs only when epithelial mesenchymal interactions are precisely replicated.

Source: Archives of Oral Biology

DOI: 10.1016/j.archoralbio.2025.106461

Authors: Pei-Jung Wu, Mar Jovani-Sancho

Institution: Universidad Cardenal Herrera CEU

Language: English

Topic: dental development and anomalies

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $3,760