Year: 2025
Type: article
Abstract: Chickpea, serves as high protein pulse crop for vegetarian people, faces major field losses from biotic stress like Fusarium wilt. Wilt management through biotechnological interventions using next generation sequencing method like transcriptomics have shown potentials. Transcriptomic data provide differential expressed genes (DEGs)that gives insights into the molecular responses in resistance mechanism. In our previous work, transcriptomic analysis has revealed many upregulated DEGs that may be promising candidates for defense during early hours of fusarium infestation. In this study we have analyzed a group of genes that highlights the importance of callose metabolism in disease management. Specifically, callose synthases genes, including Ca CalS5 , Ca CalS3, CaCalS 9X1, and Ca CalSS10 showed notable upregulation during infection in resistant genotype with the exception of Ca CalS9X2 andCa CalS7 which were upregulated in susceptible genotype. We have determined effective callose synthase complex activity during Fusarium attack in early time points and found its vital role during defense. Additionally, differential expression of genes ( Endoglucanase 24 , and Endoglucanase 12 ) involved in callose degradation were found upregulated in susceptible genotype, emphasizing their role in xylem vessel clogging together with overgrowth of mycelia structures as shown in anatomical pictures obtained from scanning electron microscopy. Confocal microscopy using aniline dye confers the callose deposition and xylem vessel blocking.
Source: Plant Growth Regulation
Authors: Kritika Sharma, Pooja Yadav, Manoj Kumar
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: hybrid
Estimated APC paid: $3,690
OpenAlex ID: W4416401070