Work

Detection of Rare and Low-expressing CAR-T cells with BD® 1-step CAR Detection Reagent 9175

Year: 2025

Type: article

Abstract: Description Flow cytometric identification and analysis of CAR-expressing cells is applied in many stages of early drug discovery and translational research. However, surface CAR protein expression can vary widely, making accurate identification and flow cytometric analysis of CAR+ cells difficult. BD® has developed 1-step reagents for resolving CAR-expressing cells using flow cytometry that are compatible with multiple fluorochrome options to accommodate varying panel needs and instrument constraints. Here, we show that our CAR Detection Reagents specifically stain CAR-expressing CHO, Jurkat CAR-T, and primary human CAR-T cells. In addition to in vitro cell culture settings, our CAR Detection Reagents performed well on lysed whole blood and in simple multi-color panels and showed compatibility with intracellular staining workflows. CAR-T cells added to donor PBMC were detected by this reagent at < 0.1% proportions, showing potential value in research studies on CAR-T persistence. Importantly, background staining was minimal on CAR-negative blood cell populations, and CARlo cells could be resolved from negative cells. Overall, our data indicate that these new reagents address the challenges of CAR+ cell detection and will empower CAR cell research. For Research Use Only. Not for use in diagnostic or therapeutic procedures. BD and the BD logo are trademarks of Becton, Dickinson and Company. © 2024 BD. All rights reserved. NPM-5437. Topic Categories Tumor Immunology: Checkpoints, Prevention, and Treatment (TIPT)

Source: The Journal of Immunology

DOI: 10.1093/jimmun/vkaf283.2613