Dietary allergen induces trafficking of CXCR5-expressing peripheral leukocytes to the central nervous system in subclinically sensitized individuals 2944
Year: 2025
Type: article
Abstract: Description Food allergen consumption has been suspected to influence mood and behavior in individuals who are sensitized but tolerant to the food. Using a mouse model of subclinical cow’s milk allergy, we previously showed that C57BL/6J mice sensitized to β-lactoglobulin (BLG, Bos d 5) exhibited behavioral changes after consuming a whey-containing diet for 2 weeks. Cortical demyelination, neuroinflammation, and CD45+ cell infiltration were also evident in the brain. Hypothesizing that allergen consumption would trigger gut-brain communication through chemokine-mediated leukocyte trafficking, we performed flow cytometric immunoprofiling of CD45+ cells in the brain. After mice were orally sensitized to BLG and placed on the whey-containing diet for 2 weeks, single-cell preparations from the whole brain were stained for immune cell markers and chemokine receptors. We observed significant increases in CXCR5+CD3+ and CXCR5+CD19+ cells in BLG-sensitized mice compared to naïve or sham-sensitized mice. Immunofluorescence staining of brain sections showed that the differences in the number of CXCR5+ cells among the groups were most prominent in the cortical regions. The adoptive transfer of BLG-sensitized donor splenocytes resulted in the trafficking of CXCR5+ donor cells to the dura mater of naïve recipients, supporting the involvement of CXCR5+ in allergen-induced peripheral-to-central leukocyte trafficking. The role of CXCR5+ leukocytes in neurobehavioral pathology is yet to be elucidated. Funding Sources Supported by NIH grant 1R01AI168563 Topic Categories Neuroimmunology (NEUR)
Source: The Journal of Immunology
Institution: University of North Dakota
Language: English
Subfield: Immunology and Allergy
Field: Medicine
Domain: Health Sciences
Open Access status: bronze
OpenAlex ID: W4416447099
FWCI:
Cites:
Cited by:
Related to: