Work

Dynamic Hydrogel Drug Delivery of mRNA for Enhancing Tertiary Lymphoid Structure Formation and Maturation 2080

Year: 2025

Type: article

Abstract: Description Tertiary lymphoid structures (TLSs) are lymphoid-like aggregates within the tumor microenvironment, linked to improved cancer prognosis and enhanced immune responses, including immune checkpoint blockade (ICB) therapies. However, inducing mature TLSs is challenging. This study aims to develop a hydrogel drug delivery system to stimulate TLS formation and maturation. We synthesized a hydrogel using 4-(4-chlorophenyl)pyridine-modified hyaluronic acid (HA-CPP) and cucurbit[8]uril (CB[8]). Loaded with cytokines CXCL13 and LIGHT or mRNA-encapsulating liposomes, the hydrogel was injected into cancer-bearing mice. TLS formation and maturation were assessed through immunofluorescence staining, and tumor growth and survival rates were evaluated. We also explored the combined effect of the hydrogel and anti-PD1 ICB therapy on tumor suppression. A single injection of HA-CPP Funding Sources The research was supported by the National Key Research and Development Program of China (2021YFA0910700). Topic Categories Tumor Immunology: Cellular Responses and Tumor Microevironment (TIME)

Source: The Journal of Immunology

DOI: 10.1093/jimmun/vkaf283.045

Authors: Chaiyaporn Kuwentrai, Weilong Tang, Matthew J Webber, Zhou Ye, Jiandong Huang

Institutions: University of Hong Kong, University of Notre Dame, Chinese Academy of Sciences, Shenzhen Institutes of Advanced Technology

Language: English

Topic: Hydrogels: synthesis, properties, applications

Subfield: Molecular Medicine

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: bronze

Funders: National Institutes of Health, Japan Society for the Promotion of Science, National Key Research and Development Program of China, National Institute of Allergy and Infectious Diseases