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Smart Integration of Lyophilized B and T cell Panels in Multiomic Analysis: Enhanced Resolution and Cost Efficiency in Immune Profiling Using Microwell-based CITEseq assay 3267

Year: 2025
Type: article
Abstract: Description High-throughput single-cell RNA sequencing (scRNA-seq) is essential for profiling complex cell populations. While traditional scRNA-seq captures RNA data only, advances with DNA-barcoded antibodies now enable simultaneous mRNA and protein analysis, providing a richer multiomic view. This often requires high sequencing depth, especially for large panels with redundant markers or high-density antigens monopolizing reads. Ambient unbound antibody noise during cell capture can also reduce data precision. To address these challenges, we developed 30-plex lyophilized T and B cell panels for targeted immune response profiling. These panels support marker “drop-ins” as needed, with minimal performance impact, and can be used independently or combined. Integrating essential lineage markers, the panels utilize selective signal attenuation with unconjugated antibodies to reduce sequencing demands for high-density antigens, enabling detection of low-abundance proteins and lowering sequencing costs. A microwell-based single-cell capture method, combined with post-capture buffer washes, minimizes background noise and enhances protein resolution, a clear advantage over droplet-based approaches. This modular, adaptable design provides cost-effective, high-resolution multiomic profiling, allow researchers to customize immune profiling to their needs.BD and the BD Logo are trademarks of Becton, Dickinson and Company. © 2024 BD. All rights reserved. NPM-5257. Topic Categories Technological Innovations in Immunology (TECH)
DOI: 10.1093/jimmun/vkaf283.1085
Authors: Elham Hatami, Hye-Won Song, Jody Martin, Ricelle Acob, Aruna Ayer, Larry Wang
Language: English
Open Access status: bronze
OpenAlex ID: W4416449639
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