Year: 2025
Type: article
Abstract: Description EditCo unveils a breakthrough in T-cell engineering: a rapid platform delivering premium-quality edited human CD4+ and CD8+ T-cells with unprecedented efficiency. The platform achieves knock-out efficiency greater than 80% in less than 2 weeks, with edited cell pools maintaining viability above 70% before cryopreservation. These cells can be thawed and expanded at least 30-fold over 14 days independent of TCR stimulation, with no loss in editing efficiency. The platform’s versatility has been demonstrated across multiple unique gene targets and diverse donor populations. Notably, edited CD4+ T-cells retain robust functionality after cryo-recovery, producing IL2, IFNγ, and TNFα upon stimulation. Similarly, edited CD8+ T-cells maintain their endogenous antigen-specific cytotoxic activity, successfully express lentiviral CAR-T constructs, and demonstrate bi-specific engager-mediated cell killing. This comprehensive validation establishes EditCo’s platform as a versatile, high-performance solution for T-cell editing, maintaining cellular integrity and functionality while delivering consistent results across multiple genetic targets and donor populations. Topic Categories Technological Innovations in Immunology (TECH)
Source: The Journal of Immunology
Authors: Paolo Piazza, Daniel Orozco, John Latimer, Alexander Brown
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: bronze
OpenAlex ID: W4416449866
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