PCR optimization for buccal swab-derived samples: overcoming sporadic inhibition with bovine serum albumin
Year: 2025
Type: article
Abstract: BACKGROUND: Buccal swabs are widely used as a non-invasive method for genomic DNA collection in large-scale genotyping studies. However, the sporadic presence of PCR inhibitors within these samples may hinder PCR amplification and affect assay reliability. This study investigates the incorporation of bovine serum albumin (BSA) into the PCR reaction mixture as an additive to counteract PCR inhibition. RESULTS: In our high-throughput setting, the incorporation of BSA significantly improved robustness, lowering failure rates to 0.1% in subsequent routine operation across 1,000,000 buccal swab samples. Despite minor challenges with foaming during automated liquid handling, no detrimental effects on PCR performance were observed. CONCLUSION: These results underscore the efficacy of BSA in improving PCR robustness, enhancing the reliability of high-throughput molecular diagnostic assays.
Source: BMC Genomics
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $3,090
OpenAlex ID: W4416453449