Investigating key genes and molecular mechanisms of prostate cancer and coronary heart disease through transcriptomics and experimental validation
Year: 2025
Type: article
Abstract: Background: Prostate cancer (PC), a common male urogenital malignancy, and coronary heart disease (CHD), a cardiovascular disease from coronary lesions causing myocardial ischemia, interact in comorbidity. This study integrated their transcriptome data to reveal comorbid mechanisms and develop cross-disease targets. Methods: In this research, candidate genes were derived from differential analysis and intersection analysis. Subsequently, machine learning algorithms were integrated with receiver operating characteristic (ROC) curve assessment and expression confirmation to identify key genes. Nomograms were further constructed, and analyses were carried out on the subcellular and chromosomal localization, enrichment pathways, molecular regulatory networks, and immune infiltration of these key genes. Potential drugs were predicted and molecular docking was performed. Ultimately, to confirm whether the expression patterns of key genes in clinical samples aligned with the bioinformatics analysis results, reverse transcription quantitative polymerase chain reaction (RT-qPCR) was conducted. Results: is associated with 11 miRNAs and 6 TFs. They are also involved in immune cell regulation. The study predicted 22 drugs, and molecular docking confirmed that they stably bound with potential drugs (e.g., DL-175) (docking score ≤-5 kcal/moL). RT-qPCR results were consistent with bioinformatics analyses (P<0.05). Conclusions: This study determined the key genes related to PC and CHD, providing new bases and targets for diagnosis, treatment, and drug development.
Authors: Ning Wu, Hongchang Gu, Zuo Qi, Zhiqiang Zhao, Lijun Cheng, Yong Wang, Zihao Liu, Tong Liu
Language: English
Subfield: Pulmonary and Respiratory Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: diamond
OpenAlex ID: W4416455058