Work

Advanced electrochemical biosensing of pathogens: Harnessing the antimicrobial properties of Ib-M peptides for highly sensitive bacterial detection

Year: 2025

Type: article

Abstract: This study describes the development of electrochemical biosensors with high sensitivity to detect pathogenic bacteria, including Escherichia coli O157:H7, Pseudomonas aeruginosa, and Staphylococcus aureus, in aqueous environments. The biosensors employ the antimicrobial peptides Ib-M1 and Ib-M6 as biorecognition elements, immobilized on gold nanoparticle-modified screen-printed electrodes via a self-assembled monolayer. Detection was achieved through electrochemical impedance spectroscopy, achieving remarkably low limits of detection of 1.4 CFU/mL for E. coli O157:H7 and S. aureus, and 0.8 CFU/mL for P. aeruginosa. The biosensors exhibited linear detection ranges of 0-100 CFU/mL for E. coli O157:H7 and S. aureus, and 0-75 CFU/mL for P. aeruginosa. Notably, the incorporation of carbon nanotubes significantly improved analytical sensitivity of the biosensors, particularly for E. coli O157:H7 and S. aureus. These results highlight the potential of the proposed biosensors for rapid, on-site monitoring of microbial contamination in drinking water, food processing environments, and clinical settings.

Source: PLoS ONE

DOI: 10.1371/journal.pone.0337227

Authors: J.L. Ropero-Vega, Y.J. Galvis-Curubo, J. M. Flórez-Castillo

Institutions: University of Magdalena, Universidad De Santander

Language: English

Topic: Electrochemical sensors and biosensors

Subfield: Electrical and Electronic Engineering

Field: Engineering

Domain: Physical Sciences

SDGs: Clean water and sanitation, Good health and well-being

Open Access status: gold

Estimated APC paid: $2,382