Effects of Silver Nanoparticle‐Loaded 18β‐Glycyrrhetinic Acid on P2X7 Receptor and Endoplasmic Reticulum Stress‐Mediated NLRP3 Inflammasome Activation in Testicular Tissue in an Animal Diabetes Model
Year: 2025
Type: article
Abstract: ABSTRACT Diabetes, a metabolic disease characterized by high blood sugar, causes damage to many organs in the body. Accordingly, the present study investigates the effects of silver nanoparticle‐loaded 18β‐glycyrrhetinic acid (AgNP+18β‐GA) on P2X7 receptor and ER stress‐mediated NLRP3 inflammasome activation in testicular tissue. 42 Wistar‐Albino rats aged 6 months were used. The rats were divided into the following seven groups: Control, AgNP+18β‐GA100, DM, DM‐18β‐GA100, DM‐AgNP+18β‐GA50, DM‐AgNP+18β‐GA100, and DM‐AgNP. After the experiment, sperm samples obtained from the rats were evaluated. Oxidative stress markers, serum IL‐1β, and TNF‐α levels were determined in the testicular tissue samples taken separately. In addition, Caspase‐1, NLRP3, P2X7, Caspase‐3, NF‐κB, IRE1, ATF6, and CHOP protein expression levels were evaluated using Western blot analysis of histopathological examinations. Specifically, the most significant decrease was observed in NLRP3, Caspase‐1, P2X7, and NF‐κB levels in the DM‐AgNP+18β‐GA100 group ( p < 0.05). About sperm parameters, while sperm motility and viability significantly decreased in diabetic groups ( p < 0.05), a significant improvement in these values was observed in the AgNP+18β‐GA applied groups ( p < 0.05). AgNP+18β‐GA improved sperm morphology and histopathological damage in diabetes‐induced testicular damage, as well as inhibited P2X7 receptor and endoplasmic reticulum stress‐mediated NLRP3 inflammasome activation.
Source: Food Science & Nutrition
DOI: 10.1002/fsn3.70893
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $3,630
Funder: Kafkas Üniversitesi
OpenAlex ID: W4416533191