Work

Chemical Stability and Bond Strength of Endodontic Sealers Exposed to Acidic, Alkaline and Protein‐Rich Environments: An In Vitro Investigation

Year: 2025

Type: article

Abstract: ABSTRACT The sealing performance of endodontic sealers may be influenced by environmental factors such as pH and blood‐derived proteins. This in vitro study evaluated the effects of pH variation and protein contamination on calcium ion (Ca 2+ ) release and push‐out bond strength (BS) of a calcium silicate‐based sealer (Bio‐C Sealer) and an epoxy resin‐based sealer (AH Plus). Standardised bovine root dentine discs were immersed for 28 days in phosphate‐buffered saline (PBS) at pH 5, 7 or 12, with or without 10% fetal bovine serum (FBS). Bio‐C Sealer showed greater Ca 2+ release and conductivity than AH Plus, with enhanced ion release in acidic and reduced in alkaline pH. Both sealers exhibited decreased BS in non‐neutral pH, though Bio‐C Sealer was more affected by FBS. These findings suggest that pH and protein exposure significantly alter sealer performance, with bioceramic materials being more sensitive to environmental changes than epoxy resin‐based sealers.

Source: Australian Endodontic Journal

DOI: 10.1111/aej.70038