Work

The Effect of Different Hydroxyapatite Structures on the Cellular Behavior of Polycaprolactone/Sodium Alginate/Collagen Scaffolds to Proliferate Mesenchymal Cells

Year: 2025

Type: article

Abstract: ABSTRACT This study demonstrates how a hydroxyapatite/(HaP) structure influences and determines cell viability/proliferation on electrospun hybrid polycaprolactone/sodium‐alginate/collagen scaffolds when mesenchymal cells are seeded. Four different HaPs were tested: (1) nanometric/(nHaP), (2) micrometric/(μHap), (3) functionalized/(nHaP f ), and (4) natural‐decellularized bovine condyle (NKB). Electrochemical, morphological, physicochemical, and biological assays were used to characterize the hybrid scaffolds. The NKB‐containing scaffold demonstrated not only the best adhesion and proliferation cell culture but also strong cellular unity, encouraging the formation of a stable biologic interface between the cells and the scaffold with a homogeneous distribution throughout the multilayered mats. The NKB compound, which contains different compositions, such as nitrogen, demonstrated the highest impedance during the entire testing (168 h) together with the rougher surface and the smallest crystallite size. On the contrary, the μHap‐containing scaffold showed the lowest metabolic activity among all samples with heterogeneous cell culture. The X‐ray photoelectron spectra demonstrated that the nitrogen content of the NKB significantly modified the polycaprolactone structure to form more CO/CN bonds, which increased the scaffold cellular viability. The biological assays were assessed using Ki‐67 immunostaining, resazurin and methodologies to evaluate the toxicity and adhesion properties of the scaffolds. The NKB‐scaffold promises optimal biocompatibility features for tissue restoration.

Source: Surface and Interface Analysis

DOI: 10.1002/sia.70031

Authors: M. Hernández, P. González‐Alva, M. F. López‐Medina, S. Rodil, A. Tejeda, K. K. Gómez

Institutions: Universidad Nacional Autónoma de México, Mexican Social Security Institute

Language: English

Topic: Bone Tissue Engineering Materials

Subfield: Biomedical Engineering

Field: Engineering

Domain: Physical Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $3,580