Work

Protocol to establish rice root aphid colonies and experiments in greenhouse settings

Year: 2025

Type: article

Abstract: The rice root aphid (RRA) Rhopalosiphum rufiabdominalis (Sasaki) is a cosmopolitan species that attacks the root system of many plants grown in fields and greenhouses, including hydroponic production systems. Here, we present a protocol to establish consistent RRA colonies used to infest plants and to set up experiments in greenhouse settings. We describe steps for researching and understanding RRA colonies and establishing and maintaining the colony. We then provide suggestions for conducting studies in a greenhouse setting.

Source: STAR Protocols

DOI: 10.1016/j.xpro.2025.104222

Authors: Nuris M. Acosta, Jonathan Lee-Rodriguez, Luis Canas

Institutions: The Ohio State University, Purdue University West Lafayette

Language: English

Topic: Insect-Plant Interactions and Control

Subfield: Insect Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

SDG: Zero hunger

Open Access status: gold

Estimated APC paid: $1,550