Work

Insights into cannabinoid receptor 2 (CB2) anterograde trafficking and pharmacological chaperoning

Year: 2025

Type: article

Abstract: Cannabinoid Receptor 2 (CB 2 ) is a promising therapeutic target for modulating inflammation. Canonical signalling responses to receptor ligands are critically dependent on cell surface receptor expression. However, it is also now appreciated that intracellular G protein-coupled receptors can contribute to signalling responses and influence functional outcomes. Therefore, understanding how the subcellular distribution of receptors is controlled is also highly pertinent. CB 2 is observed to be expressed at the cell surface as well as having a considerable proportion expressed intracellularly. Despite this distribution being well established, little is known about the regulation of CB 2 anterograde trafficking and subcellular distribution. We report that sustained treatment with a range of CB 2 agonists and inverse agonists stimulates a distinct population of CB 2 to be delivered to the cell surface, at various expression levels and despite agonists concurrently internalising cell surface CB 2 . We present evidence that this ligand-stimulated anterograde trafficking is a result of CB 2 agonists, as well as inverse agonists, acting as pharmacological chaperones. We also report that a di-lysine (KK) motif in the CB 2 C-terminal tail is required for basal delivery to the cell surface. Corroborating the hypothesis that CB 2 ligands can act as pharmacological chaperones, sustained CB 2 ligand stimulation induces cell surface expression of the mutated receptor and alters maturation states as measured by western blotting. Our finding that prolonged exposure to CB 2 ligands can induce CB 2 cell surface delivery via pharmacological chaperoning may well have important implications for optimal design of CB 2 -targeted therapeutics.

Source: Cellular and Molecular Life Sciences

DOI: 10.1007/s00018-025-05961-w

Authors: Caitlin R. M. Oyagawa, Braden Woodhouse, Karren C. Wood, Michelle Glass, Natasha L. Grimsey

Institutions: University of Auckland, Maurice Wilkins Centre, University of Otago

Language: English

Topic: Cannabis and Cannabinoid Research

Subfield: Pharmacology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $4,290