Work

Protocol for in vitro phagocytosis assay for primary mouse microglia and human embryonic stem cell-derived microglia

Year: 2025

Type: article

Abstract: Microglia, the resident immune cells of the brain, maintain brain health by clearing detrimental debris, including amyloid-β (Aβ). Here, we present a protocol for assessing Aβ uptake by primary mouse microglia and human embryonic stem cell-derived microglia using an in vitro phagocytosis assay. We describe procedures for pHrodo-labeled oligomeric Aβ treatment and real-time signal detection using the Incucyte SX5 system. We also detail steps for microglia culture, Aβ labeling, and quantification of phagocytosis over time. For complete details on the use and execution of this protocol, please refer to Zhu et al. 1

Source: STAR Protocols

DOI: 10.1016/j.xpro.2025.104219

Authors: Beika Zhu, Alicia L. Thurber, Xianhua Piao

Institution: University of California, San Francisco

Language: English

Topic: Neuroinflammation and Neurodegeneration Mechanisms

Subfield: Neurology

Field: Neuroscience

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,550