Improving electrochemical aptasensor sensitivity for Bacillus cereus spore detection in food safety applications
Year: 2025
Type: article
Abstract: Rapid detection of Bacillus cereus spores is critical for preventing food contamination and spoilage. Many existing methods detect B. cereus cells instead of spores, and cannot be applied directly in foods. Here, combination of aptamers that bind different moieties at the surface of B. cereus spore with rapid electrochemical detection is presented. When different DNA aptamers were immobilized on screen printed gold electrode, they showed higher binding capacity for B. cereus spores than individual aptamers suggesting a synergic effect. The biosensor exhibited a wide dynamic range (10 2 - 10 7 CFU/mL) of low limits of detection (∼1 CFU/mL) using just 15 μL of sample. Validation in spiked salad, using direct spore sensing in rinse water and comparing it to the culturing method, confirmed its sensitivity and specificity. This aptamer-combined approach achieving the rapid (15 min) and single-step detection may also be suitable for detecting other foodborne pathogens.
Source: Talanta
Authors: Milica Sentic, Francesco Rizzotto, Zorica Novakovic, Aleksandar Karajic, Brahim Heddi, Jasmina Vidic
Institutions: AgroParisTech, Université Paris-Saclay, Microbiologie de l’alimentation au service de la santé, University of Belgrade, Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement, Institute of Chemistry, Technology and Metallurgy, University of Novi Sad, BioSense Institute, Tufts University, Institut des Sciences Moléculaires, Centre de recherche Paul Pascal, École Normale Supérieure Paris-Saclay, Centre National de la Recherche Scientifique, Laboratoire de Biologie et Pharmacologie Appliquée
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $4,610
Awards: G1446243131, MONITORING AND DETECTION OF BIOTIC AND ABIOTIC POLLUTANTS BY ELECTRONIC, PLANTS AND MICROORGANISMS BASED SENSORS, Sensitive peptIde- and apta-assays for on-site dEtectioN of bActerial cells and spores in milk and meat samples, G8234278901, Integration of PAper-based Nucleic acid testing mEthods into Microfluidic devices for improved biosensing Applications
OpenAlex ID: W4416582956