Year: 2025
Type: article
Abstract: With monoclonal antibodies becoming one of the largest classes of biopharmaceuticals, it is important to have curated data to train computational models that can accelerate their design. Despite the massive amount of mutagenesis data generated on antibody-antigen interactions, only a few small, well-curated datasets are available. This paper introduces AbAgym, a manually curated repository comprising approximately 324k mutations in antibody-antigen complexes, including approximately 10% of interface mutations, whose effects on antibody-antigen binding have been experimentally quantified through deep mutational scanning (DMS) experiments. We collected and curated 68 DMS datasets from the literature together with the three-dimensional structure of each antibody-antigen complex. We benchmarked the performance of established force field methods as well as recent machine learning models that predict the change in binding affinity upon mutation. The former achieved modest performance, whereas the latter performed only marginally better than random. Finally, our analysis of hotspot residues responsible for immune evasion highlights the importance of accounting for biological complexities, such as conformational changes or oligomeric states that influence antibody-antigen binding, which are often overlooked. Abagym is freely available for academic use at https://github.com/3BioCompBio/Abagym.
Source: mAbs
Institutions: Université Libre de Bruxelles, San Sebastián University
Language: English
Subfield: Radiology, Nuclear Medicine and Imaging
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $3,575
OpenAlex ID: W4416599557