Work

A DNAzyme amplifier-based immunoassay for small molecule detection

Year: 2025

Type: article

Abstract: , thus generating amplified fluorescence signals. In the presence of small molecule targets, the targets compete with the antigen-labeled DNAzyme for binding with the immobilized antibodies on the microplate, resulting in a decrease in the fluorescence signal. We successfully detected small molecules, digoxin and folic acid, at picomolar levels through the proposed immunoassay. The immunoassay can specifically identify small molecules and enable their detection in complex sample matrices. This strategy provides a simple and sensitive approach to selectively detect small molecules, inspiring researchers to develop DNAzyme-based immunoassays with multiple functions.

Source: The Analyst

DOI: 10.1039/d5an01013f

Authors: Han Pang, Qiang Zhao

Institutions: Chinese Academy of Sciences, Research Center for Eco-Environmental Sciences, University of Chinese Academy of Sciences, State Key Laboratory of Environmental Chemistry and Ecotoxicology

Language: English

Topic: Advanced biosensing and bioanalysis techniques

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Funders: Chinese Academy of Sciences, National Key Research and Development Program of China

Awards: G3371992070, G7758101378