Figure 5 from Autologous Thymic Organoids Support Functional T-cell Education and Enhance Antitumor Immunity in Humanized Mice with Melanoma Xenografts
Year: 2025
Type: other
Abstract: Changes in intratumoral heterogeneity in mHMTA PDXs can be explained by neoAg selection. A, NeoAg distribution across HLA alleles (B) Kullback–Leibler logos show enrichment of hydrophobic amino acids at position 9 in the neoAg pool. The height of each one letter amino acid code is the absolute enrichment relative to unselected peptides. Values above the x axis are positively enriched whereas those below are depleted. C, Distribution of estimated changes in variant allele frequency between the mHMTA and mHM models in all variants identified by exome sequencing. Values to the right of the dotted line represent variants that enriched, or underwent positive selection in mHMTA, whereas values to the left represent variants that underwent negative selection. D, Significant changes between mHM and mHMTA variant allele frequencies are plotted against the predicted HLA binding affinity for nine-mer peptides derived from each variant. The points are colored by which class I HLA molecule the peptide was predicted to bind. All significantly altered variants were found to produce at least two neoAgs. Error bars represent SE. E, Single letter codes for each amino acid are shown with the mutant residue outlined in each. The majority of mutations occurred at TCR contact residue locations (positions 3–8).
Authors: J. Jason Morton, Stephan A. Ramos, Nathaniel Alzofon, Stephen B. Keysar, Lucas H. Armitage, Alexander S. Baker, Jessie M. Barra, Tugs-Saikhan Chimed, Phuong N. Le, Cera Nieto, Alice N. Weaver, Carissa M. Thomas, Bettina Miller, William Robinson, Theresa M. Medina, Dexiang Gao, Holger A. Russ, Antonio Jimeno
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4416599736
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