Work

Autoantibody signatures in children with celiac disease, juvenile idiopathic arthritis, and polyautoimmunity

Year: 2025

Type: article

Abstract: Objective: To determine if multiplex autoantibody arrays can identify novel biomarker signatures in children with one or multiple autoimmune diseases (polyautoimmunity). Methods: Plasma collected from children (18 years or younger) in the Boston Children's Hospital Precision Link Biobank for Health Discovery between January 2007 and June 2021 was analyzed using a microarray with 120 autoantigens associated with various autoimmune diseases to assess for both immunoglobulin G (IgG) and A (IgA) autoantibodies. To determine if disease-specific, we binary classified those with polyautoimmunity and the most common autoimmune diseases in our cohort (autoimmune thyroid disease, type 1 diabetes, or celiac disease [CeD]). For each comparison within and between groups, the autoantibody intensity was releveled to the control and a linear model was fit. Results: = 32). Overall, children with polyautoimmunity had higher levels of specific autoantibodies relative to the other groups. Children with CeD and polyautoimmunity expressed higher levels of H/K ATPase IgA and IgG and MDA5 IgG compared to those with CeD alone. Conclusions: Use of multiplex antigen arrays in patients with and without polyautoimmunity is a valuable tool to identify known and potentially novel disease-antigens. Further study is needed to determine the role of H/K ATPase autoantibodies in monitoring of CeD patients.

Source: JPGN Reports

DOI: 10.1002/jpr3.70119