The role of chitosan in modulating physicochemical and biological behavior of solid lipid nanoparticles
Year: 2025
Type: article
Abstract: Chitosan, a cationic polysaccharide known for its peculiar properties, plays a key role in enhancing biological interactions at mucosal surfaces. This study investigated the role and impact of chitosan on physicochemical and biological properties of Cyclosporine A -loaded solid lipid nanoparticles (SLN). Three types of SLN were developed: SLN devoid of chitosan, chitosan-associated SLN during hot homogenization (CH-SLN), and chitosan-coated SLN (CH-c-SLN). Physicochemical characterization revealed differences in particle shape, with chitosan presence increasing particle size and zeta potential. SAXS/WAXS and DSC studies demonstrated that Cyclosporine A and chitosan influenced lipid organization and thermal behavior. Moreover, mucoadhesion and mucin interaction were stronger in chitosan-associated SLN (CH-SLN), as evidenced by SAXS and ITC, due to chitosan cationic nature. At last, preclinical studies revealed enhanced cellular uptake in presence of chitosan. These findings demonstrated that chitosan-associated SLN enhanced mucoadhesion and cellular interaction while maintaining structural integrity, providing a promising platform for drug delivery.
Authors: Marco Ruggeri, Marta Pollini, Caterina Ricci, Barbara Vigani, Eleonora Bianchi, Silvia Rossi, Elena Del Favero, Giuseppina Sandri
Institutions: University of Pavia, University of Milan
Language: English
Subfield: Pharmaceutical Science
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $4,390
Award: G8169032545
OpenAlex ID: W4416643136