Peer Review Report For: RNAtor: an Android-based application for biologists to plan RNA sequencing experiments [version 1; peer review: 1 approved with reservations, 1 not approved]
Year: 2017
Type: peer-review
Abstract: RNA sequencing (RNA-seq) is a powerful technology that identifies novel transcripts (coding, non-coding and splice variants), understands transcript structures, and estimates gene/allele expression. Biologists face specific challenges while designing RNA-seq experiments. The nature of these challenges lies in determining the total number of sequenced reads and replicates required for detecting marginally differentially expressed transcripts, and in determining the adequate number of lanes to use in a sequencing flow cell. Despite previous attempts to address these challenges, easily accessible and biologist-friendly mobile applications do not exist. Thus, we developed RNAtor , a mobile application for Android platforms, to aid biologists in correctly designing their RNA-seq experiments. The recommendations from RNAtor are based on simulations and real data.
Source: F1000Research
Author: Niranjan Nagarajan
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $1,350
OpenAlex ID: W4416648695