Get started
Work

Corrigendum to “A renal biopsy-anchored multi-marker signature involving AOPEP SNP-driven splicing, miR-27b-3p and glycated albumin for stratifying renal damage in type 2 diabetes”. [DIAB 229 (2025) 112460]

Year: 2025
Type: erratum
Abstract: Aims: Stratifying renal damage in type 2 diabetes is challenging due to overlapping features between diabetic nephropathy (DN) and non-diabetic renal disease (NDRD). While miR-27b-3p modulates kidney fibrosis in DN through the lysine63 ubiquitination pathway, its upstream regulation and diagnostic relevance remain unclear. Methods: In a biopsy-verified cohort of 231 chronic kidney disease (CKD) patients with and without type 2 diabetes, we investigated whether AOPEP promoter SNPs drive alternative splicing affecting intronic miR-27b-3p expression. We also assessed the diagnostic utility of combining these biomarkers with clinical parameters, such as glycated albumin (GA), to distinguish DN from NDRD. Results: The rs10761364 minor allele was associated with reduced urinary miR-27b-3p and AOPEP splicing that excluded exons hosting the miR-23b/27b/24 cluster. This pattern, observed in vivo and under hyperglycemia in vitro, led to elevated AOPEP protein isoforms. A model combining rs10761364, GA, and urinary miR-27b-3p showed high diagnostic accuracy (AUC up to 0.93) in discriminating DN from NDRD. miR-27b-3p inversely correlated with GA, and GA-based models outperformed those using HbA1c. Conclusion: We identify a genotype- and glucose-dependent mechanism regulating miR-27b-3p via AOPEP splicing and propose a biopsy-anchored, non-invasive biomarker panel (rs10761364, GA, miR-27b-3p) to differentiate DN from NDRD, supporting personalized nephrology care.
DOI: 10.1016/j.diabres.2025.113009
Authors: Francesca Conserva, Francesco Pesce, Claudia Cinefra, Tommaso Maria Marvulli, Ighli Di Bari, Alessandra Stasi, Maria Felicia Faienza, Michele Rossini, Adriano Montinaro, Elena Squiccimarro, Giorgia Sclavo, Annalisa Schirinzi, Francesca Di Serio, Viji Nair, Damian Fermin, Rajasree Menon, Edgar Otto, Fabio Sallustio, Anna Gallone, Giovanni Stallone, Gianluigi Zaza, Luigi Laviola, Marco Fiorentino, Francesco Giorgino, Maria F. Gomez, Matthias Kretzler, Loreto Gesualdo, Paola Pontrelli
Institutions: University of Bari Aldo Moro, Agostino Gemelli University Polyclinic, Istituti di Ricovero e Cura a Carattere Scientifico, Azienda Universitaria Ospedaliera Consorziale - Policlinico Bari, University of Michigan, University of Foggia, University of Calabria, Lund University
Language: English
Subfield: Nephrology
Field: Medicine
Open Access status: hybrid
Estimated APC paid: $4,410
OpenAlex ID: W4416655073
Cites:
Cited by:
Related to: