GM-CSF and specific type 2 cytokines induce CD103+ and CD301b+ cell states in cDC1s and cDC2s
Year: 2025
Type: article
Abstract: The heterogeneity of conventional dendritic cells type 1 (cDC1s) and type 2 (cDC2s) is well established, yet the identity and origin of CD301b + cDC2s remain debated. Here, we show that CD301b + cDC2s and CD103 + cDC1s develop from pre-committed progenitors in response to granulocyte/macrophage colony-stimulating factor (GM-CSF). While CD103 + cDC1s acquire their phenotype and functional properties through GM-CSF-driven differentiation from pre-cDC1s, CD301b + cDC2s emerge as cytokine-induced states from DC2- and DC3-committed progenitors. CD103 + cDC1s and CD301b + cDC2s exhibit enhanced T cell priming capacities and distinct cytokine expression profiles upon GM-CSF exposure. In vivo , DC-intrinsic GM-CSF sensing is dispensable for acquiring CD103 and CD301b expression with the notable exception of lung DCs, while specific type 2 cytokines induce CD103 and CD301b ex vivo . These findings identify GM-CSF and specific type 2 cytokines as central regulators of cDC1 and cDC2 effector differentiation and establish CD301b as a marker of a cytokine-driven cDC2 state.
Source: Cell Reports
Authors: Lukas Amon, Damir Vurnek, Anna Seichter, Giorgi Tchitashvili, Tomasz Kaszubowski, Markus Mroz, Nincy Debeuf, Tina Vogler, Nicole Küpper, Kaushikk Ravi Rengarajan, Lukas Lächele, Nounagnon R. Tochoedo, Anna Baranska, Stella E. Autenrieth, Falk Nimmerjahn, Kai Hildner, Klaus Pfeffer, Barbara U. Schraml, Lukas Heger, Bart N. Lambrecht, Christian H.K. Lehmann, Diana Dudziak
Institutions: Friedrich-Alexander-Universität Erlangen-Nürnberg, Ghent University, Universitätsklinikum Erlangen, VIB-UGent Center for Inflammation Research, Jena University Hospital, Friedrich Schiller University Jena, Heinrich Heine University Düsseldorf, Institut für Immunologie, Ludwig-Maximilians-Universität München, German Cancer Research Center, Erasmus MC, Erasmus University Rotterdam, Center for Sepsis Control and Care
Language: English
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $5,620
Awards: G101308371, G1064716527, G1284162446, G131901245, G1479737578, Regulierung der ilealen Immunantwort im Rahmen der Überwachung von Darmkrebs durch das Immunsystem:
DAMPs, MAMPs und Selbstantigene intestinaler Stammzellen, G195474297, G2329111812, G281949974, Immunmodulatorische Funktion von Inflammasomen in humanen primären dendritischen Zellen, G2843771166, G374510482, TRR 359: Perinatale Entwicklung der ImmunzellTopologie (PILOT), TRR 374: Tubulussystem und Interstitium der Niere: (Patho)Physiologie und Crosstalk, G5376099354, KFO 5024: ImmunCheckpoints der Kommunikation zwischen Darm und Gehirn bei entzündlichen und neurodegenerativen Erkrankungen (GB.com), G5810831391, GRK 2504: Neue antivirale Strategien: von der Chemotherapie bis zur Immunintervention, TRR 221: Steuerung der TransplantatgegenWirt und TransplantatgegenLeukämieImmunreaktionen nach allogener Stammzelltransplantation, G6496555215, EXC 2051: Balance of the Microverse, TRR 305: Über die Analyse der metastatischen Koloniebildung zu neuen systemischen Krebstherapien, G6958885147, G7369019519, G7377979947, Understanding Heterogeneity of Eosinophils in Airway Disease
OpenAlex ID: W4416663369