Year: 2025
Type: article
Abstract: Stress granules (SGs) are essential cytoplasmic, membraneless organelles that form in response to cellular stress, functioning to prevent mRNA translation and protect mRNA from damage. However, the mechanism of SG formation remains largely unknown. Here, utilizing a systems-level technique for quantification of RNA cap epitranscriptome, we found that mRNA Cap1 and non-canonical caps are predominantly enriched in SGs, with the composition of mRNA caps in SGs of mammalian cells differing between different stress conditions. Knockdown of RNA guanine-7-methyltransferase (RNMT) and phosphorylated CTD interacting factor 1(PCIF1) both resulted in substantial changes in the content and composition of mRNA caps and RNMT knockdown caused failure of SG formation under different stress conditions. Furthermore, proteomic, Co-IP and confocal immunofluorescence analysis of these knockdown cells and SGs revealed that mRNAs partition into SGs through cap-protein interactions. These findings collectively revealed the significant role of mRNA cap in SG formation and stress response in mammalian cells.
Authors: Mingxin Guo, Ying Feng, Guantong Qi, Wenran Ma, Xiaoli He, Ruiqi Li, Yu Liu, Jin Wang
Institution: Inner Mongolia University
Language: English
Topic: RNA modifications and cancer
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $4,290
Funders: Innovative Research Group Project of the National Natural Science Foundation of China, National Natural Science Foundation of China, Fudan University, Inner Mongolia University, Natural Science Foundation of Inner Mongolia, Inner Mongolia Collaborate Innovation Cultivate Center for Castor, Ten Thousand Talent Plans for Young Top-notch Talents of Yunnan Province, Natural Science Foundation for Young Scientists of Shanxi Province, Young Scientists Fund, State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry
OpenAlex ID: W4416665650