Work

Co-Culture of Monascus purpureus and Aspergillus niger Isolated from Wuyi Hongqu to Enhance Monascus Pigments Production While Inhibiting Citrinin Production

Year: 2025

Type: article

Abstract: Wuyi Hongqu (WYH), also called black-skin-red-koji, which has been utilizing as a fermentation starter for more than one thousand years in China, is a symbiotic combination of Monascus spp. and Aspergillus niger formed through long-term application and domestication. In this study, the strains of Monascus purpureus and A. niger isolated from WYH samples were used to investigate their mutual influence, especially the effects on three main secondary metabolites from M. purpureus, Monascus pigments (MPs), monacolin K (MK), and citrinin (CIT), using a double-sided Petri dish (DSPD). The results showed that co-cultivation of M. purpureus and A. niger strains was favorable to increase the MPs production while inhibiting the CIT production by M. purpureus, especially when M. purpureus strains (M1-1 or M9) were co-cultivated with certain A. niger strains (An1-2 or An9), respectively, and both Monascus strains hardly produced detectable CIT. The expression levels of CIT-related genes in M. purpureus M1-1 or M9 were greatly restricted when co-cultivated with A. niger An1-2 or An9 confirmed by RT-qPCR. This study provides important insights into the selection of WYH production strains and the effects of fungal interactions.

Source: Journal of Fungi

DOI: 10.3390/jof11120829

Authors: Qin Yu, Xi Yuan, Fusheng Chen

Institutions: Huazhong Agricultural University, Wuhan Business University

Language: English

Topic: Microbial Metabolism and Applications

Subfield: Biotechnology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $3,137

Funder: Wuhan Business University