Work

Rapid Identification and Characterization of Pathogenic Bacteria Isolates Using Fluorescence Excitation Spectroscopy and Spectral Deconvolution

Year: 2025

Type: article

Abstract: Bacterial detection and identification are crucial tasks in today's world due to increasing casualties and severity caused by bacteria in hospital-acquired infections, food-borne infections, etc. This warrants an urgent need for rapid detection and identification of different bacterial strains. Fluorescence spectroscopy is a widely used technique for bacteria characterization owing to its high sensitivity, real-time, and simple measurement capabilities. In this work, fluorescence excitation spectra of 200 bacteria isolates belonging to eight different species were acquired under identical conditions with 25 isolates per species. The fluorescence excitation spectra were subjected to spectral deconvolution to extract four Gaussian components viz., tryptophan in protein under buried and exposed conditions, nucleic acids and anthranilic acid. Linear discriminant analysis applied to normalized fluorescence excitation spectra of 200 samples classified the bacterial strains with 100% accuracy using leave-one-out cross-validation.

Source: Journal of Biophotonics

DOI: 10.1002/jbio.202500408

Authors: Anandh Sundaramoorthy, Bharanidharan Ganesan, Aruna Prakasarao, Singaravelu Ganesan

Institutions: Anna University, Chennai, Hindustan Institute of Technology and Science

Language: English

Topic: Spectroscopy Techniques in Biomedical and Chemical Research

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funders: Department of Science and Technology, Ministry of Science and Technology, India, University Grants Commission, Board of Research in Nuclear Sciences

Awards: G2280149095, G3525898281, G5440021229