Work

Anti-inflammatory activity of Clonostachys rosea culture medium extract in LPS-stimulated RAW 264.7 macrophage cells

Year: 2025

Type: article

Abstract: This study evaluated the anti-inflammatory potential of a Clonostachys rosea culture medium extract (CR).To assess its bioactivity, lipopolysaccharide-stimulated RAW 264.7 macrophages were employed as an in vitro inflammation model.Cytotoxicity testing revealed that CR exhibited no toxicity at concentrations of 12.5, 25, and 50 μg/mL.In contrast, CR markedly suppressed the production of nitric oxide and prostaglandin E 2 in a dose-dependent manner.Consistently, the expression levels of inducible nitric oxide synthase and cyclooxygenase-2 were significantly reduced, indicating effective inhibition of inflammatory mediator synthesis.Furthermore, CR treatment downregulated pro-inflammatory cytokines, including tumor necrosis factor-α, interleukin-1β, and interleukin-6, supporting its potent anti-inflammatory activity.Collectively, these findings suggest that CR possesses promising pharmacological potential as a natural anti-inflammatory agent. Keywords Anti-inflammatory •

Source: Journal of Applied Biological Chemistry

DOI: 10.3839/jabc.2025.060

Authors: Byeong-Min Choi, Kyeong-Mi Lee, Hyehyun Hong, Seung-Young Kim

Institution: Sun Moon University

Language: English

Topic: Phytochemistry and Biological Activities

Subfield: Plant Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: diamond

Funder: Sun Moon University