Work

Distinct roles of glycocalyx components in regulating endothelial functions in a perfused three-dimensional human endothelium-on-a-chip

Year: 2025

Type: article

Abstract: Using a perfused 3-D human endothelium-on-a-chip, we investigated the endothelial glycocalyx (EGX) in vascular regulation. EGX degradation was linked to endothelial dysfunction induced by dengue NS1, but not by TNF-α. In HUVECs, heparan sulfate (HS) degradation increased permeability, whereas sialic acid (SA) cleavage had the opposite effect. SA degradation, but not HS, enhanced monocyte adhesion in HUVECs and human coronary ECs. This study highlights distinct, component-specific roles of HS and SA in regulating vascular function.

Source: American Journal of Physiology-Cell Physiology

DOI: 10.1152/ajpcell.00191.2025

Authors: Tanotnon Tanawattanasuntorn, Anunyaporn Phungsom, Kenjiro Muta, Jeerapa Lokakaew, Nunya Chotiwan, Pimonrat Ketsawatsomkron

Institutions: Mahidol University, Ramathibodi Hospital, Ramathibodi Chakri Naruebodindra Hospital

Language: English

Topic: Proteoglycans and glycosaminoglycans research

Subfield: Cell Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: hybrid

Funder: Mahidol University