Work

Repurposing the Sanger Reagent for Optical Sensing of the Concentration and Enantiomeric Ratio of Chiral Amines, Amino Alcohols, and Amino Acids

Year: 2025

Type: article

Abstract: The well-known, readily available Sanger reagent is used for quantitative chiroptical sensing of the amount and enantiomeric composition of amines, amino alcohols, and amino acids. A practical assay that is fast, adaptable to a variety of solvents, and only requires mixing of the sample and probe in the presence of triethylamine prior to the CD and UV measurements is introduced. The analyte tagging generates characteristic CD and UV signals at 405 and 345 nm, respectively, which allow accurate concentration and er analysis without any workup or tedious sample preparation.

Source: Chirality

DOI: 10.1002/chir.70062

Authors: F. Safia Kariapper, Austin U. Wolf, Christian Wolf

Institution: Georgetown University

Language: English

Topic: Molecular spectroscopy and chirality

Subfield: Spectroscopy

Field: Chemistry

Domain: Physical Sciences

Open Access status: hybrid

Estimated APC paid: $4,030

Funders: National Science Foundation, Georgetown University, Division of Chemistry