On-chip EPR spectrometry of metalloproteins using superconducting lumped element resonators
Year: 2025
Type: article
Abstract: (four dip-pen layers). Two reference bulk samples were pipetted from the same solution onto the remaining two resonators. The microwave transmission of the device, measured at 11 mK, shows evidence of the coupling of protein spins to the photon excitations of all resonators. In particular, the resonance broadening measured as a function of magnetic field provides the spin resonance absorption spectrum. The analysis suggests that proteins tend to self-orient on the chip. It also allows estimating the single spin to single photon coupling strength, which is around 9 Hz. This high coupling value suggests that dip-pen nanolithography gives rise to a close to optimum interface between the molecules and the chip surface. The developed methodology combines an increase in sensitivity of at least three orders of magnitude with the ability to characterize multiple samples in a single experiment, opening the door to a highly sensitive multi-analyte detection technology.
Source: Nanoscale
DOI: 10.1039/d5nr03119b
Institutions: University of the Basque Country, Universidad de Zaragoza, Instituto Biofisika, Instituto de Nanociencia y Materiales de Aragón, Consejo Superior de Investigaciones Científicas, Centro de Astrobiología, Madrid Institute for Advanced Studies, IMDEA Nanoscience, Fundacion Agencia Aragonesa para la Investigacion y el Desarrollo
Language: English
Subfield: Biophysics
Domain: Life Sciences
Open Access status: hybrid
Awards: G1175115578, G1446647517, G1658930240, G2015000536, G2262748287, G24120701, G2601397997, G2858269097, G2914759765, G294895050, G300979063, G3480869486, G3532318254, G3681454997, G4042783231, G410571670, G4146472935, G425668148, G451917667, G4617935805, G5080475149, G5195188938, G523076202, G5539308778, G5606506422, G5967599077, G6033112984, G6061596652, G7043789982, G7069624064, G7084143925, G983705713
OpenAlex ID: W4416697377