Work

Automated Syntheses of Xylan Oligosaccharides Containing β3-Linkages Enable Substrate Specificity Studies of Xylanases from Marine Bacteria

Year: 2025

Type: article

Abstract: Marine polysaccharides, including β3-xylan and mixed-linkage xylan (MLX), play a central role in the ocean's carbon cycle. Using an optimized xylose donor, automated glycan assembly enabled the synthesis of β3- and MLX-oligosaccharides up to decasaccharides. Interrogation of these structurally defined substrates with the marine xylanases Xyl4, AlXyn26A, and MfXyn26A revealed a surprisingly broad substrate scope of MLXases toward both β3-xylan and MLX motifs, highlighting AGA-derived glycans as powerful probes for studying marine polysaccharide metabolism.

Source: Organic Letters

DOI: 10.1021/acs.orglett.5c04433

Authors: Nitish Verma, Nils Rustmeier, Uwe Osswald, Fabian Pfrengle

Institution: BOKU University

Language: English

Topic: Carbohydrate Chemistry and Synthesis

Subfield: Organic Chemistry

Field: Chemistry

Domain: Physical Sciences

Open Access status: hybrid

Estimated APC paid: $4,500

Funder: HORIZON EUROPE European Research Council