Work

The phytochemical analysis and biological activities assessment of secondary metabolites in callus cultures of Clitoria ternatea L.

Year: 2025

Type: article

Abstract: The butterfly pea is a significant source of bioactive secondary metabolites. Callus cultures provide a viable option for the reliable synthesis of important secondary metabolites, overcoming the limitations of inconsistent yields from field-grown plants. The present results indicated that MS medium with 1.0 mg/L BAP and 1.0 mg/L 2,4-D produced a highly significant growth stimulation for callus induction in Clitoria ternatea L. after 40–45 days under darkness. The biomass of the callus augmented with each subsequent subculture, reaching its peak by the sixth subculture. The macronutrient content, antioxidant assays, flavonoids, phenolics, and HPLC analysis were assessed. Anti-diabetic, anticancer, and anti-inflammatory characteristics were evaluated. According to the HPLC analysis, the callus culture of C. ternatea L. comprises a range of flavonoid and phenolic compounds that demonstrated the most anti-diabetic, anti-inflammatory, and cytotoxic effects. In conclusion, using the callus culture of C. ternatea L. has shown promise in secondary metabolite synthesis. Moreover, callus cultures of C. ternatea L. have significant nutritional value, which increases antioxidant activity, in addition to their potential application as new natural secondary metabolites.

Source: Plant Cell Tissue and Organ Culture (PCTOC)

DOI: 10.1007/s11240-025-03281-2

Authors: Dina Mostafa Mohammed, Walla M. A. Abdelazeez, Ahmad A. Suliman, Ahmed G. M. Sief-Eldein, Gamil R. Aboueldis

Institutions: National Research Centre, Al-Azhar University, Desert Research Center

Language: English

Topic: Plant tissue culture and regeneration

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Zero hunger

Open Access status: hybrid

Estimated APC paid: $3,890