Work

MiR‐33‐5p‐opioidergic Signaling Regulates Cognitive Impairment Induced by Bile Duct Ligation in Rats: Ameliorative Role of Naloxone

Year: 2025

Type: article

Abstract: OBJECTIVE: The present study investigated the involvement of miR-33-5p-opioidergic signaling in the regulation of cognitive impairment induced by bile duct ligation (BDL) in rats, with an emphasis on the ameliorative role of naloxone. METHODS: Among the four groups of Wistar rats (control, sham, BDL, and BDL + Naloxone [Nalx]), the common bile duct was occluded only in the BDL groups. Fourteen days after BDL induction and following completion of the analysis of the liver function tests of alkaline phosphatase, alanine aminotransferase, aspartate transaminase, direct bilirubin, total bilirubin, gamma-glutamyl transpeptidase (GGT), and lactate dehydrogenase, the cognitive tests and electrophysiological field potential responses were recorded. Then, the hippocampus was assessed for the expression level of miR-33-5p using PCR. Ultimately, a histopathological study of the liver and hippocampus was carried out. RESULTS: The findings revealed that except for GGT (p = 0.1935), all liver function tests were elevated (P = 0.0001) following BDL. The impaired inhibitory avoidance memory (P < 0.0001) and plasticity (P < 0.0001) and the hippocampal overexpression of miR-33-5p following BDL (P <0.0001) were ameliorated by Nalx. In a qualitative histopathological study of the liver and hippocampus, Nalx attenuated the BDL-induced changes. CONCLUSIONS: It is concluded that the BDL-mediated dysfunction has a molecular, electrophysiological, and histopathological basis and might be regulated via miR-33-5p and opioidergic signaling. The study emphasizes the potential ameliorative role of Nalx on biliary and nervous system pathologies.

Source: Brain and Behavior

DOI: 10.1002/brb3.71105

Authors: Mohadeseh Rahimi‐vala, Behrang Alani, Ali Arjmand, Tahere Mazoochi, Abolfazl Ardjmand

Institutions: Kashan University of Medical Sciences, University of Guilan

Language: English

Topic: MicroRNA in disease regulation

Subfield: Cancer Research

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $3,630