Work

Genetic analysis of potential biomarkers and therapeutic targets in hypoxia and pyroptosis from psoriasis

Year: 2025

Type: article

Abstract: Background: Pyroptosis and hypoxia significantly influence the onset and progression of psoriasis. However, their interactions remain unclear. Therefore, this study aimed to elucidate the roles of pyroptosis-related genes (PRGs) and hypoxia-related genes (HRGs) in psoriasis. Methods: Psoriasis-related datasets, PRGs and HRGs were incorporated. Firstly, differentially expressed genes (DEGs) between psoriasis and control were identified. PRGs and HRGs expressions were utilized to calculate PRGs and HRGs scores, which were used to find key module genes.Then, candidate genes were acquired by taking the intersection of the previously obtained DEGs and key module genes. Furthermore, biomarkers were selected with machine learning algorithms, gene expression analyses, and receiver operating characteristic (ROC). A nomogram was constructed and verified. Further analyses conducted to explore the potential mechanism. Finally, expression of biomarkers was evaluated by real-time reverse transcriptase-polymerase chain reaction (RT-qPCR). Results: PI3 and LCE3D, which had significantly higher expression and an area under the curve (AUC) ＞0.9, were ascertained as biomarkers. Nomogram, constructed by biomarkers, predicted the risk of psoriasis well.Then, cytosolic DNA-sensing pathway, focal adhesion, and oxidative phosphorylation were enriched for the 2 biomarkers. Immune infiltration analysis showed that 20 types of cells were significantly different between psoriasis and control. In addition, 18 potential drugs were predicted by biomarkers. Finally, RT-qPCR showed that biomarkers were higher in psoriasis. Conclusion: This study identified 2 biomarkers (PI3 and LCE3D) associated with pyroptosis and hypoxia in psoriasis. These findings offer valuable insights that could inform the treatment of psoriasis.

Source: Biochemistry and Biophysics Reports

DOI: 10.1016/j.bbrep.2025.102314

Authors: Jing Cui, Nianyi Zhang, Liuyi Yang, Xiaoping Shen, Ming Ni

Institution: Affiliated Hospital of Guizhou Medical University

Language: English

Topic: Psoriasis: Treatment and Pathogenesis

Subfield: Immunology

Field: Immunology and Microbiology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $2,700