Protocol to study social transmission of stress in group-housed mice using restraint stress
Year: 2025
Type: article
Study design: Study Protocol
Abstract: Here, we present a protocol to study social transmission of stress in group-housed mice using restraint stress. We describe steps for exposing mice to restrained conspecifics separated by an acrylic divider to induce indirect stress responses. We detail procedures for acute and chronic paradigms, followed by behavioral, physiological, and immunological assessments in both restrained and bystander mice. The protocol allows for the investigation of sensory and social mechanisms underlying stress contagion, while excluding visual cues. For complete details on the use and execution of this protocol, please refer to Horvath et al. 1 • Protocol for inducing social stress transmission in group-housed mice • Guidance on performing acute and chronic restraint stress paradigms • Steps for collection of blood, organs, and lymphoid tissues • Assessment of behavioral, physiological, and immunological responses Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Here, we present a protocol to study social transmission of stress in group-housed mice using restraint stress. We describe steps for exposing mice to restrained conspecifics separated by an acrylic divider to induce indirect stress responses. We detail procedures for acute and chronic paradigms, followed by behavioral, physiological, and immunological assessments in both restrained and bystander mice. The protocol allows for the investigation of sensory and social mechanisms underlying stress contagion, while excluding visual cues.
Source: STAR Protocols
Authors: Dennis Mink, Dennis Horvath, Michael Basler
Language: English
Subfield: Behavioral Neuroscience
Field: Neuroscience
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $1,550
OpenAlex ID: W4416745179