Work

Development of a Taqman-based real time PCR method for the detection of the banded cricket (Gryllodes sigillatus) in feed

Year: 2025

Type: article

Abstract: The demand for sustainable protein sources has led to a growing interest in the use of insects in animal feed. This study focuses on the detection of banded crickets ( Gryllodes sigillatus ) in feed using a real time PCR method. The research aims to address the challenges associated with identifying insects in feed, in light of European regulations governing the use of processed animal proteins. The methodology involved the development of a specific probe, to be combined with a previously developed primer pair. Method’s specificity, efficiency and sensitivity were internally validated. The technical detection limit was 4 pg of DNA/reaction. The method was able to successfully detect target species G. sigillatus at a concentration of 0.1% in various model feed samples. The results indicate that the developed real time PCR method reliably detects G. sigillatus in feed, providing a means to ensure compliance with regulatory requirements. Judging from similar cases, the method appears to be transferable to food analysis, subject to verification of practical sensitivity, especially in ultra-processed foods. This research contributes to ongoing efforts to promote the use of insects as a sustainable source, emphasizing the importance of accurate species identification in the context of food safety and regulatory compliance.

Source: Journal of Food Composition and Analysis

DOI: 10.1016/j.jfca.2025.108670

Authors: Finja König, Sebastian Broll, Jutta Zagon, Hermann Broll, Cristiano Garino

Institution: Federal Institute for Risk Assessment

Language: English

Topic: Identification and Quantification in Food

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Zero hunger

Open Access status: hybrid

Estimated APC paid: $3,450