Enhanced proteome profiling of human cerebrospinal fluid using a commercial plasma enrichment strategy
Year: 2025
Type: article
Abstract: Cerebrospinal fluid (CSF) is a valuable liquid biopsy for identifying protein biomarkers in neurological diseases, yet its proteome profiling faces challenges due to the large dynamic range of protein abundances. In this study, we assessed the effectiveness of a commercial enrichment strategy, initially developed for plasma samples, in enhancing the detection of low-abundance proteins in human CSF. We demonstrate significant improvements in protein identification and coverage depth while maintaining high reproducibility and low coefficients of variation. These findings underscore the potential of this enrichment strategy to facilitate rapid and sensitive CSF analysis, advancing biomarker discovery in neurological research.
Source: Journal of Proteomics
Language: English
Subfield: Spectroscopy
Field: Chemistry
Domain: Physical Sciences
Open Access status: hybrid
Estimated APC paid: $3,570
Funders: Ministerio de Ciencia, Innovación y Universidades, International Centre for Theoretical Sciences, European Commission, Generalitat de Catalunya, Ministerio de Ciencia e Innovación, Fundación Bancaria Caixa d'Estalvis i Pensions de Barcelona, Horizon 2020 Framework Programme, European Research Council, Instituto de Salud Carlos III, European Regional Development Fund, H2020 Marie Skłodowska-Curie Actions
Awards: G1350557659, G1482478015, G3836421357, G3866471055, Junior Leader la Caixa Postdoctoral Fellowship Programme: Shaping the new generation of leaders in research, G4619558077, G505100453, Computational Proteomics Training European Innovative Network, G7049930438, G7685134749, El impacto clínico de los nuevos biomarcadores sanguíneos de Alzheimer: el estudio PLASMAR, Factores sanguíneos pro-rejuvenecimiento y pro-envejecimiento en la enfermedad de Alzheimer preclínica y su efecto en la estructura cerebral y la cognición, G8800515849
OpenAlex ID: W4416756001