Work

Sustainable valorization of Pleurotus pulmonarius via green dual-enzyme technology: Novel optimization for functional protein hydrolysates

Year: 2025

Type: article

Abstract: This research established a sustainable dual-enzyme hydrolysis method for generating nutrient-dense hydrolysates from grey oyster mushrooms (Pleurotus pulmonarius), a native resource of Vietnam. Process optimization integrated a quadratic regression model using response surface methodology (RSM) and an artificial neural network combined with a genetic algorithm (ANN–GA). The objective was to enhance nitrogen recovery, amino acids, peptides, glutamic acid, and lysine, while minimizing NH 3 formation. ANN–GA demonstrated superior predictive accuracy compared to RSM, with RMSE values of 1–2 % versus 2–5 %. In contrast, RSM offered precise quantitative insights regarding the effects of factors. The optimal conditions (50.9 °C, 6.45 h, pH 6.12) resulted in a nitrogen recovery of 27.23 %, with 6.926 g/100 g of amino acids, 11.41 g/100 g of peptides, 6.398 g/100 g of glutamic acid, 0.0845 g/100 g of lysine, and 0.0089 g/100 g of NH 3 . Levels of essential amino acids were comparable to or higher than those documented in previous studies. The sustainability of the method not only reduces waste and benefits farmers but also provides a practical route to producing mushroom-based functional food and seasoning ingredients due to their high amino acid and peptide content. • First ANN–GA and RSM optimization of dual-enzyme hydrolysis for P. sajor-caju . • Maximized peptides and essential amino acids with reduced ammonia content. • ANN–GA showed higher prediction accuracy than RSM in process optimization. • Green processing of Vietnamese mushrooms adds value for local farmers. • Hydrolysate suitable for functional foods and seasoning applications

Source: Food Chemistry X

DOI: 10.1016/j.fochx.2025.103310

Authors: Nguyen Thi Ngoc Giang, Tran Van Khai

Institutions: Vietnam National University Ho Chi Minh City, An Giang University

Language: English

Topic: Protein Hydrolysis and Bioactive Peptides

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDGs: Responsible consumption and production, Zero hunger

Open Access status: gold

Estimated APC paid: $2,330