Gut Microbiota Modulation by Lacticaseibacillus paracasei EFEL6501 Ameliorates Muscle Atrophy
Year: 2025
Type: article
Abstract: Probiotics play a crucial role in promoting host health by modulating the composition of the gut microbiota through the production of their own bioactive metabolites.The aim of the study was to investigate the anti-atrophic effects of Lacticaseibacillus paracasei EFEL6501 (EFEL6501) in dexamethasone (DEX)-treated C2C12 myotubes and a mouse model.In vitro experiments demonstrated that specific bioactive metabolites present in the cell culture supernatant (CS) and lysate supernatant (LS) of EFEL6501 alleviated muscle degradation and restored muscle protein synthesis in DEXinduced C2C12 myotubes.Similarly, EFEL6501 supplementation in mice significantly enhanced muscle thickness (6.09 mm), grip strength (117.87 g), and the cross-sectional area (CSA) (34.11 μm²) of the gastrocnemius muscle, compared to the DEX group (5.70 mm, 106.87 g and 29.79 μm², respectively), by suppressing protein degradation pathways and improving muscle differentiation.Furthermore, EFEL6501 positively modulated the gut microbiota composition by increasing the abundance of beneficial bacteria, including Lactobacillus reuteri (7.19%), Bifidobacterium choerinum (25.66%),Bacteroides uniformis (0.29%), Allobaculum (0.63%), and Faecalibaculum (18.00%) compared to the DEX group (3.44%, 0.75%, 0.14%, -0.63%, and 8.53%, respectively), while also elevating acetate concentrations from 1.57 ± 0.27 mM to 1.97 ± 0.16 mM.Taken together, EFEL6501 may serve as a potential functional probiotic for preventing muscle atrophy by regulating muscle metabolism and gut microbiota composition.
Institutions: Chungbuk National University, Ildong (South Korea)
Language: English
Subfield: Food Science
Domain: Life Sciences
Open Access status: bronze
Funders: National Research Foundation, Chungbuk National University, Ministry of Education, Ministry of Agriculture, Food and Rural Affairs, Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries, National Research Foundation of Korea, Ministry of Education, India
OpenAlex ID: W4416758195