Figure 3 from MUC1-C Dictates PBRM1-Mediated Chronic Induction of Interferon Signaling, DNA Damage Resistance, and Immunosuppression in Triple-Negative Breast Cancer
Year: 2025
Type: other
Abstract: MUC1, PBRM1, and IRF1 regulate similar gene sets that are shared with TNBC tumors. A, RNA-seq was performed in triplicate on BT-549 cells silenced for MUC1, PBRM1, and IRF1. The datasets were analyzed for effects of MUC1-C silencing on repressed and activated genes as depicted by the Volcano plots. B, Venn diagram depicting the overlap of 196 downregulated genes in BT-549 cells silenced for MUC1, IRF1, and PBRM1 (Supplementary Table S3). C, RNA-seq datasets from BT-549 cells silenced for MUC1, IRF1, and PBRM1 were analyzed with GSEA for enrichment distribution using the indicated IFN-related gene signatures. D, Heatmaps of 72 common genes between the HALLMARK INTERFERON GAMMA RESPONSE and HALLMARK INTERFERON ALPHA RESPONSE signatures in BT-549 cells silenced for MUC1, IRF1, and PBRM1. The row indicator shows the 45 common genes from the 196 DEGs identified in B (yellow, Supplementary Table S4). E, Common up- and downregulated IFN response genes (detectable 66 genes out of 72 genes) in MUC1-high versus MUC1-low TNBC tumors from the METABRIC and TCGA-BRCA datasets. The outline (gold/gray) represents common DEGs in BT549 cells identified in D. The red/gray/blue dot indicates significantly up/downregulated genes (MUC1high vs. MUC1low) in one or both of the TCGA-BRCA/METABRIC cohorts.
Authors: Nami Yamashita, Yoshihiro Morimoto, Atsushi Fushimi, Rehan Ahmad, Atrayee Bhattacharya, Tatsuaki Daimon, Naoki Haratake, Yuka Inoue, Satoshi Ishikawa, Masaaki Yamamoto, Tsuyoshi Hata, Sayuri Akiyoshi, Qiang Hu, Tao Liu, Henry Withers, Song Liu, Geoffrey I. Shapiro, Tomoharu Yoshizumi, Mark D. Long, Donald Kufe
Language: English
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4416764850