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Kjellmaniella crassifolia for the Prevention of Ultraviolet B-Induced Oxidative Stress in HaCaT Keratinocytes

Year: 2025

Type: article

Abstract: ethanol extract (KCE) in UVB-exposed HaCaT keratinocytes. The cytotoxicity/cytoprotective activity of KCE was assessed using MTT, and DCF-DA assays to measure cell viability and intracellular reactive oxygen species (ROS), respectively. Apoptotic cell death was examined through nuclear staining, Annexin V/PI assays, and mitochondrial membrane potential analysis via JC-1 staining. Western blot results indicated that KCE suppressed the mitochondrial-mediated apoptotic pathway, reduced phosphorylation of MAPK and NF-κB, and activated the Nrf2/HO-1 antioxidant signaling pathway. Overall, these findings demonstrate that KCE effectively attenuates UVB-induced oxidative stress and apoptosis, highlighting its potential as a natural bioactive ingredient for skin protection in cosmeceutical applications.

Source: Journal of Microbiology and Biotechnology

DOI: 10.4014/jmb.2508.08041