Year: 2025
Type: article
Abstract: Snapdragon (Antirrhinum majus L.) represents a commercially significant ornamental species .In this study, a shoot regeneration protocol was developed using hypocotyl explants from ten snapdragon varieties.The experimental materials included four cut flower varieties (Maryland Apple Blossom, Maryland True Pink, Maryland White, and Maryland Red) and six bedding varieties (Snapshot White, Snapshot Rose, Snapshot Yellow, Snapshot Pink, Twinny Peach, and Twinny Apple Blossom).To achieve consistent and high-frequency shoot regeneration, hypocotyl explants were cultured on media with different combinations of NAA (0.05, 0.1, and 0.2 mg.L -1 ), Zeatin (2 and 3 mg.L -1 ), and AgNO 3 (4 mg.L -1 ).Across six varieties, the combination of 0.05 mg.L -1 NAA, 3 mg.L -1 Zeatin, and 4.0 mg.L -1 AgNO 3 resulted in 100% shoot regeneration efficacy and the highest number of shoots per explant.However, three bedding varieties (Snapshot Rose, Snapshot Yellow, Snapshot Pink) consistently exhibited low shoot numbers per explant across all tested media combinations.Therefore, to improve the regeneration rate and shoot number, explants from these varieties were further cultured on media containing 0.05 mg.L -1 NAA, 2 mg.L -1 Zeatin, and different concentrations of AgNO 3 (0, 1, 2, 3, and 4 mg.L -1 ).Interestingly, the absence of AgNO 3 produced the highest shoot numbers per explant and achieved 100% shoot regeneration frequency.The effect of AgNO 3 on shoot regeneration varied among varieties, acting as either a positive or a negative regulator.Among the four combinations of rooting media tested, the Maryland White variety showed enhanced rooting in the absence of NAA.Conversely, Snapshot White (0.5 mg.L -1 NAA) and Twinny Peach (0.1 mg.L -1 NAA) demonstrated improved rooting with NAA supplementation.
Source: Journal of Plant Biotechnology
Institutions: Sunchon National University, Kyungpook National University
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
SDG: Zero hunger
Open Access status: diamond
Funder: Sunchon National University
Award: G1356601628
OpenAlex ID: W4416774117
FWCI:
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