Work

Long-wavelength oblique back-illumination microscopy for deep in vivo imaging

Year: 2025

Type: article

Abstract: Oblique back-illumination microscopy (OBM) is a label-free imaging technique that captures differential forward scattering in reflection mode to generate high-contrast pseudo-transmission images of cells and microvessels. While OBM benefits from multiple light scattering to detect forward-scattered signals, its imaging depth is constrained by tissue scattering between the objective lens and the imaging plane. In this study, we introduce a long-wavelength OBM system operating at 1650 nm—significantly longer than previous implementations—to mitigate scattering effects and extend imaging depth. Compared to a similar system using an 800 nm light source, our 1650 nm OBM achieves markedly deeper in vivo imaging of the mouse brain. This advancement in high-contrast, deep-tissue imaging holds promise for more detailed investigations into the pathophysiology of living biological systems.

Source: Biomedical Optics Express

DOI: 10.1364/boe.579269

Authors: Ye-Chan Cho, Jin Hee Hong, Sungsam Kang, Wonjun Choi, Wonshik Choi, Yookyung Jung

Institutions: Korea University, Albert Einstein College of Medicine

Language: English

Topic: Optical Imaging and Spectroscopy Techniques

Subfield: Radiology, Nuclear Medicine and Imaging

Field: Medicine

Domain: Health Sciences

Open Access status: gold

Estimated APC paid: $1,913

Funder: National Research Foundation of Korea