Work

Development of intestinal tissue-resident T cells in early-life mice before and after weaning

Year: 2025

Type: article

Abstract: Intestinal tissue-resident T cells have been poorly characterized during early-life. Here, we phenotypically characterized tissue-resident (intravascular staining negative) CD4 + and CD8α + T cells in spleen and intestine (lamina propria and intraepithelial lymphocytes), in suckling and juvenile mice before and after weaning, respectively. While minor changes were observed in spleen T cells, significant changes in tissue-resident T cells frequency and phenotype were identified after weaning. Although differences were seen between CD4 + and CD8α + T cells in lamina propria and intraepithelial lymphocytes, in general, an increase of CCR7 - CD44 hi , CD69 + , CD103 + , and P2X7R + tissue-resident T cells were detected in the post-weaning period. Our study highlights the association of weaning with the development of intestinal tissue-resident T cells in early-life and suggests that dietary and microbial shifts during weaning play a crucial role in shaping tissue-resident T cell compartments.

Source: Scientific Reports

DOI: 10.1038/s41598-025-26848-7

Authors: Catherine Montenegro, Siyuan Ding, Federico Perdomo-Celis, Manuel A. Franco

Institutions: Pontificia Universidad Javeriana, Washington University in St. Louis

Language: English

Topic: T-cell and B-cell Immunology

Subfield: Immunology

Field: Immunology and Microbiology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $2,690